Eastern blotting siv rau dab tsi?
Eastern blotting siv rau dab tsi?

Video: Eastern blotting siv rau dab tsi?

Video: Eastern blotting siv rau dab tsi?
Video: Tshuaj theem vim li cas thiaj tau nqi ?/ Siv kho mob dab tsi?/paris polyphylla use for? 2024, Kaum ib hlis
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Cov sab hnub tuaj blot , los yog sab hnub tuaj blotting , yog cov txheej txheem biochemical siv txhawm rau txheeb xyuas cov protein tom qab kev hloov pauv hloov pauv (PTM) nrog rau kev sib ntxiv ntawm lipids, phosphates, thiab glycoconjugates. Nws yog feem ntau siv txhawm rau txheeb xyuas cov carbohydrate epitopes.

Ib yam li ntawd, koj tuaj yeem nug, dab tsi yog blotting siv rau?

Ib sab qaum teb blot yog ib txoj kev kuaj siv rau tshawb xyuas cov RNA molecules tshwj xeeb ntawm kev sib xyaw ntawm RNA. Sab qaum teb blotting tuaj yeem yog siv rau txheeb xyuas ib qho piv txwv ntawm RNA los ntawm cov ntaub so ntswg tshwj xeeb lossis hom cell txhawm rau ntsuas RNA kev qhia ntawm cov noob tshwj xeeb.

Tsis tas li ntawd, dab tsi yog kuaj hauv Western blotting thiab Southern blotting? A Southern blot (sau nrog lub peev "S" vim nws muaj npe tom qab British biologist Edwin Qab teb ) feem ntau yog siv rau kev tshawb pom ntawm DNA ib ntus hauv cov qauv DNA. Cov western blot yog siv los txheeb xyuas cov proteins tshwj xeeb hauv cov qauv los ntawm "immunoblotting".

Tom qab ntawd, ib tug kuj yuav nug, dab tsi yog blotting hauv biology?

Los ntawm Wikipedia, dawb encyclopedia. A blot , nyob rau hauv molecular biology thiab noob caj noob ces, yog ib txoj kev hloov cov proteins, DNA los yog RNA mus rau ib tug cab kuj (piv txwv li, ib tug nitrocellulose, polyvinylidene fluoride los yog nylon membrane).

Vim li cas tib neeg ua Southern blotting?

Southern blotting yog tsim los nrhiav ib qhov sib lawv liag ntawm DNA nyob rau hauv ib tug complex sib tov. Piv txwv li, Southern Blotting tuaj yeem siv los nrhiav cov noob tshwj xeeb hauv tag nrho genome. Tus nqi ntawm DNA xav tau rau cov txheej txheem no yog nyob ntawm qhov loj thiab cov haujlwm tshwj xeeb ntawm kev sojntsuam.

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