Video: Vim li cas spliceosome tseem ceeb?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov spliceosome catalyzes tshem tawm ntawm introns, thiab ligation ntawm flanking exons. Introns feem ntau muaj GU nucleotide ib ntus ntawm 5 'kawg splice site, thiab ib qho AG ntawm 3' kawg splice site. Muaj ntau cov proteins pom muaj zinc-binding motif, uas underscores qhov tseem ceeb zinc nyob rau hauv lub splicing mechanism.
Tsis tas li ntawd, dab tsi yog lwm txoj kev splicing thiab vim li cas nws tseem ceeb?
Lwm txoj kev splicing ntawm RNA yog txheej txheem tseem ceeb rau kev hloov cov lus qhia genomic rau hauv cov protein ua haujlwm. Nws ua lub luag haujlwm tseem ceeb hauv kev tswj hwm cov noob qhia thiab kev sib txawv ntawm cov protein nyob hauv ntau hom eukaryotes. Hauv tib neeg, kwv yees li 95% ntawm cov noob qoob loo ntau exon tau dhau los lwm txoj kev splicing.
Ib tug kuj yuav nug, leej twg nrhiav tau lub spliceosome? Tam sim no, yog tias peb tsuas muaj U1 snRNA… Nyob rau hauv ib qho tseem ceeb ntawm kev tawg, John Abelson lub chaw kuaj mob tsis ntev los no tau tsim ib qho hauv vitro splicing system thiab tom qab ntawd, los ntawm kev khiav cov khoom rho tawm ntawm sucrose gradient, txheeb xyuas qhov ∼40S particle uas muaj. 32P-labeled splicing intermediates, uas lawv dubbed lub spliceosome ” (9).
Tsis tas li ntawd, puas yog spliceosome ib qho enzyme?
Cov spliceosome yog kawg ib enzyme uas ua rau RNA substrate. Nws kuj yog ib qho RNP complex uas tau hloov zuj zus nyob ib puag ncig ntawm tsib lub RNA luv luv uas yog cov xeeb leej xeeb ntxwv ntawm ib qho qub catalytic RNA.
Puas yog spliceosome yog protein?
Cov spliceosome yog ib tug complex me me nuclear (sn)RNA- protein ntau tshuab uas tshem tawm introns los ntawm pre-mRNAs los ntawm ob tug successive phosphoryl hloov tshuaj. Rau txhua qhov kev tshwm sim splicing, lub spliceosome yog sib sau ua ke de novo ntawm lub pre-mRNA substrate thiab ib tug complex series ntawm cov kauj ruam los ua ke ua rau lub active conformation.
Pom zoo:
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