Cov txheej txheem:
Video: Dab tsi yog qhov tseem ceeb ntawm vector?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov yam ntxwv tseem ceeb tshaj plaws ntawm vectors yog:
- Lub vector yuav tsum yog DNA molecule kom nws tuaj yeem cloned nrog cov noob ntawm kev txaus siab.
- Lub vector yuav tsum muaj qhov tshwj xeeb txwv qhov chaw.
- Lub vector yuav tsum muaj tus cim xaiv tau.
- Lub vector yuav tsum muaj Ori site los ntawm qhov twg rov ua dua tuaj yeem pib.
Kuj kom paub yog, dab tsi yog cov yam ntxwv uas vectors thiab plasmids yuav tsum muaj?
Ib qho ntawm thawj yam ntxwv ntawm plasmid vectors yog tias lawv me me. Sib nrug los ntawm lawv qhov loj me, lawv yog cov cim los ntawm keeb kwm ntawm kev rov ua dua, xaiv cov cim thiab ntau qhov chaw cloning. Qhov zoo tagnrho plasmid vectors muaj cov lej luam siab hauv lub cell.
6 hom vectors yog dab tsi? Rau qhov loj ntawm vectors yog:
- Plasmid. Circular extrachromosomal DNA uas autonomously replicates hauv cov kab mob cell.
- Phaj. Linear DNA molecules muab los ntawm bacteriophage lambda.
- Cosmids.
- Kab mob Artificial Chromosomes.
- Poov Artificial Chromosomes.
- Tib neeg Artificial Chromosome.
Tom qab ntawd, peb qhov tseem ceeb ntawm plasmid yog dab tsi?
Qhov tseem ceeb ntawm Plasmid Vectors
- Rov ua dua. Replication ntawm plasmid DNA yog nqa los ntawm tib enzymes uas replicate E.
- Selectable Markers (Antibiotic resistance)
- Ntau qhov chaw cloning (los yog polylinkers)
- Ib leeg-stranded DNA ntau lawm.
- Bacteriophage txhawb nqa.
- Kev npaj me me ntawm cov kab mob Plasmid.
Dab tsi yog qhov ua haujlwm ntawm vector hauv genetic engineering?
Ib tug vector yog DNA molecule siv los nqa cov khoom siv caj ces ntxiv rau hauv a cell . Ib tug vector uas nqa txawv teb chaws DNA yog hu ua recombinant DNA.
Pom zoo:
Dab tsi yog qhov txiaj ntsig tseem ceeb ntawm kev taw qhia ntawm tes los ntawm kev sib cuag lub cev ncaj qha?
Kev taw qhia kuj tshwm sim ntawm cov hlwb uas yog kev sib cuag ntawm lub cev. Kev sib cuam tshuam ntawm cov proteins ntawm qhov chaw ntawm cov hlwb tuaj yeem ua rau muaj kev hloov pauv hauv cov cwj pwm ntawm tes. Piv txwv li, cov proteins nyob rau saum npoo ntawm T-cells thiab antigen nthuav tawm cov hlwb sib cuam tshuam los ua kom muaj kev taw qhia hauv T-cells
Dab tsi yog qhov txawv ntawm qhov sib txawv ntawm qhov sib txawv thiab qhov sib txawv ntawm qhov ntab?
Integers thiab floats yog ob yam sib txawv ntawm cov ntaub ntawv tus lej. Ib tus lej (feem ntau hu ua anint) yog tus lej tsis muaj tus lej lej. Float isa floating-point number, uas txhais tau hais tias nws yog tus lej uas muaj tus lej lej. Floats siv thaum xav tau ntau dua
Vim li cas qhov tseem ceeb ntawm qhov tseem ceeb?
Thaum koj pom tus nqi kiag li hauv qhov teeb meem lossis qhov sib npaug, nws txhais tau hais tias txhua yam nyob hauv tus nqi tiag tiag yog qhov zoo. Cov txiaj ntsig tsis zoo feem ntau siv rau hauv cov teeb meem cuam tshuam nrog kev nyob deb thiab qee zaum siv nrog qhov tsis sib xws. Qhov ntawd yog qhov tseem ceeb uas yuav tsum nco ntsoov nws zoo li qhov deb ntawm xoom
Ob qhov tseem ceeb ntawm lub voj voog ntawm tes yog dab tsi thiab dab tsi tshwm sim rau lub cell hauv txhua theem?
Muaj ob theem tseem ceeb hauv lub voj voog ntawm tes. Thawj theem yog interphase thaum lub cell loj hlob thiab replicates nws DNA. Qhov thib ob yog theem mitotic (M-Phase) thaum lub sijhawm cell faib thiab hloov ib daim qauv ntawm nws cov DNA mus rau ob lub hlwb zoo tib yam
Dab tsi yog 2 qhov tseem ceeb ntawm lub voj voog ntawm tes thiab dab tsi tshwm sim rau lub cell hauv txhua theem?
Cov xwm txheej no tuaj yeem muab faib ua ob qhov tseem ceeb: interphase (hauv nruab nrab ntawm kev sib faib theem ua pawg G1 theem, S theem, G2 theem), thaum lub xov tooj ntawm tes tsim thiab ua nrog nws cov haujlwm metabolic; mitotic theem (M mitosis), thaum lub sij hawm lub cell yog replicating nws tus kheej