Video: Vim li cas universal 16s rDNA?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Vim li cas universal 16S rDNA primers siv hauv koj qhov kev sim? A. Lawv yuav anneal rau qhov chaw khaws cia zoo heev ntawm cov noob uas encodes kab mob 16 S rRNA. Lawv yuav anneal rau cim sequences ntawm noob encoding 16 S rRNA hauv cov kab mob tshwj xeeb.
Kuj nug, 16s rDNA sequencing yog dab tsi?
16 S ribosomal ua RNA (los yog 16S rRNA ) yog ib feem ntawm 30S me me subunit ntawm prokaryotic ribosome uas khi rau Shine-Dalgarno ib ntus. Cov gene coding rau nws yog hu ua 16 S rRNA noob thiab siv nyob rau hauv reconstructing phylogenies, vim qhov qeeb ntawm evolution ntawm thaj av no ntawm cov noob.
Qhov thib ob, txoj haujlwm ntawm 16s rRNA yog dab tsi? Qhov ntev ntawm 16 S rRNA coding gene yog hais txog 1500bp, uas muaj txog 50 ua haujlwm thaj chaw. 16 S rRNA muaj tus lej muaj nuj nqi : ?Lub immobilization ntawm ribosomal proteins ua raws li scaffolding. ?3'end muaj qhov rov qab SD ntu uas siv los khi rau AUG pib codon ntawm mRNA.
Tsis tas li ntawd, vim li cas 16s rRNA gene siv sequencing?
Cov 16S ribosomal RNA noob cov lej rau RNA tivthaiv ntawm 30S subunit ntawm cov kab mob ribosome. Vim yog qhov nyuaj ntawm DNA-DNA hybridization, 16S rRNA gene sequencing yog siv raws li ib lub cuab yeej los txheeb xyuas cov kab mob ntawm hom kab mob thiab pab sib txawv ntawm cov kab mob sib xws [8].
Tib neeg puas muaj 16s rRNA?
Raws li lwm tus muaj sau tseg, 16 S rRNA noob yog * txhua yam *; cov ribosomes ua tau ' tsis txhais mRNA yam tsis muaj lawv 16 S rRNA tiv thaiv, yog li tag nrho cov kab mob muaj nws. Vim cov noob no yog tseem ceeb, lawv yog kuj heev * txuag heev *. Qhov ntawd txhais tau tias nws muaj peev xwm tsim tau tsob ntoo ntawm lub neej txuas ua ke tag nrho cov kab mob paub.
Pom zoo:
Universal genetic code txhais li cas?
1. Cov txheej txheem ntawm DNA thiab RNA cov kab ke uas txiav txim siab cov amino acid sequences siv nyob rau hauv lub synthesis ntawm lub cev cov proteins. Nws yog lub hauv paus biochemical ntawm heredity thiab ze li ntawm universal nyob rau hauv tag nrho cov kab mob
Yuav ua li cas dej yog universal hnyav?
Dej muaj peev xwm yaj ntau yam khoom sib txawv, uas yog vim li cas nws yog ib qho hnyav hnyav. Thiab, dej yog hu ua 'universal solvent' vim nws dissolves ntau tshaj li lwm yam kua. Qhov no tso cai rau cov dej molecule los ua attracted rau ntau lwm hom molecules
Yuav ua li cas yog vim li cas vim li cas lub epigenome hloov?
Kev ua neej nyob thiab ib puag ncig (xws li kev haus luam yeeb, noj zaub mov thiab kab mob sib kis) tuaj yeem ua rau tus neeg muaj kev ntxhov siab uas ua rau muaj tshuaj lom neeg. Cov lus teb no, dhau los, feem ntau ua rau muaj kev hloov pauv hauv epigenome, qee qhov tuaj yeem ua rau puas tsuaj
Puas muaj peev xwm hluav taws xob thiab lub zog muaj peev xwm tib yam Vim li cas lossis vim li cas ho tsis?
Lub zog muaj peev xwm hluav taws xob Ue yog lub zog muaj peev xwm khaws cia thaum cov nqi tawm ntawm qhov sib npaug (xws li lub zog muaj peev xwm gravitational). Lub peev xwm hluav taws xob zoo ib yam, tab sis ib tus nqi, Ueq. Ib qho hluav taws xob muaj peev xwm sib txawv ntawm ob lub ntsiab lus hu ua voltage, V = Ue2q−Ue1q
Txoj cai universal ntawm gravitation tau muab los li cas?
Txoj Cai Tswjfwm Ntiag Tug hais tias: "Txhua yam khoom ntawm huab hwm coj hauv ntiaj teb nyiam txhua yam khoom ntawm pawg nrog lub zog uas ncaj qha rau cov khoom ntawm lawv cov pawg thiab inversely proportional rau lub square ntawm kev sib cais ntawm lawv cov chaw."