Video: Puas yog DNA polymerase 3 yog Holoenzyme?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
DNA polymerase III yog a holoenzyme , uas muaj ob lub ntsiab enzymes ( Pol III), txhua qhov muaj peb lub subunits (α, ? thiab θ), zawv zawg clamp uas muaj ob beta subunits, thiab clamp-loading complex uas muaj ntau subunits (δ, τ, γ, ψ, thiab χ).
Kuj tseem paub yog, lub luag haujlwm ntawm DNA polymerase 3 yog dab tsi?
DNA polymerase III holoenzyme yog lub luag haujlwm tseem ceeb rau kev rov ua dua tshiab DNA synthesis hauv E. coli. Nws nqa tawm primer-initiated 5' rau 3 ' polymerization ntawm DNA ntawm ib leeg-stranded DNA template, thiab 3 'rau 5' exonucleolytic kho ntawm mispaired nucleotides.
Qhov thib ob, qhov txawv ntawm DNA polymerase 1 thiab 3 yog dab tsi? DNA polymerase 3 yog qhov tseem ceeb rau kev rov ua dua ntawm cov thawj coj thiab cov lagging strands whereas DNA polymerase 1 Nws yog ib qho tseem ceeb rau kev tshem tawm ntawm RNA primers los ntawm cov khoom tawg thiab hloov nws nrog cov nucleotides xav tau. Cov enzymes no tsis tuaj yeem hloov ib leeg li ob leeg muaj txawv cov haujlwm yuav tsum tau ua.
Kuj tseem paub yog, puas yog DNA polymerase III pom hauv eukaryotes?
Cov chloroplast kuj muaj DNA pol γ. Nyob rau sab saum toj ntawm pols α, δ thiab ε eukaryotes muaj ntau cov enzymes kho: pols β, η, ι, κ thiab ζ. Tsis tsuas yog peb muaj cov enzymes sib txawv tab sis eukaryotic tshuaj cov hlwb muaj cov ntawv luam ntawm cov enzymes ntau dua li prokaryotes. coli muaj 10 mus rau 20 molecules ntawm DNA pol III.
Dab tsi yog Holoenzyme?
A holoenzyme yog ib qho enzyme nrog nws cov cofactor xav tau; nws ua haujlwm zoo ib yam li ib qho enzyme. Holoenzymes tuaj yeem tsim los ntawm ntau qhov me me hu ua subunits. Lwm cov proteins tuaj yeem txuas rau enzymes los tsim ib qho holoenzyme complex.
Pom zoo:
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