Video: Arthur Kornberg nrhiav DNA polymerase li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
coli kab mob thiab radioisotope tracers, Kornberg pom tias kev sib txuas ntawm nucleotides thiab lwm yam khoom xyaw ua rau cov synthesis ceev tshaj plaws DNA . Los ntawm xyoo tom ntej nws tau pom thiab ntxuav cov enzyme tseem ceeb, DNA polymerase , los ntawm E. coli, thiab muaj peev xwm ua ke DNA hauv lab.
Ntawm no, Arthur Kornberg nrhiav tau dab tsi?
Arthur Kornberg (Lub Peb Hlis 3, 1918 - Lub Kaum Hli 26, 2007) yog ib tug neeg Asmeskas biochemist uas yeej Nobel nqi zog hauv Physiology lossis Tshuaj 1959 rau nws nrhiav pom ntawm "cov txheej txheem hauv kev sib xyaw ua ke ntawm deoxyribonucleic acid (DNA)" ua ke nrog Dr. Severo Ochoa ntawm New York University.
Ib tug kuj yuav nug, leej twg nrhiav tau DNA polymerase? Arthur Kornberg
Nyob rau hauv txoj kev no, thaum twg Arthur Kornberg nrhiav tau DNA polymerase lub molecule uas yog lub luag hauj lwm rau assembling qhov chaw ntawm DNA thaum replication?
Kornberg yog feem ntau paub zoo rau nws nrhiav pom thiab purification ntawm DNA polymerase los ntawm Escherichia coli, ib qho enzyme uas nws thiab nws cov npoj yaig tau qhia yog instrumental hauv lub synthesis ntawm DNA . Tshaj tawm hauv Xyoo 1956, txoj haujlwm no tau tsim thawj zaug uas DNA replication yog tsav los ntawm ib qho enzyme.
Dab tsi enzyme tau Arthur Kornberg cais tawm?
Matthew Meselson thiab Franklin Stahl tau tsim cov txheej txheem ntawm kev ceev gradient centrifugation thiab siv qhov no los ua pov thawj tias DNA yog replicated semi-conservatively. Arthur Kornberg txheeb xyuas thiab cais DNA polymerase I - ib qho ntawm cov cov enzymes uas tuaj yeem hloov DNA.
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