Cov txheej txheem:
Video: Peb kauj ruam tseem ceeb ntawm kev rho tawm DNA los ntawm dos yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Peb theem pib ntawm kev rho tawm DNA yog 1) lysis, 2) nag lossis daus , thiab 3) purification. Hauv cov kauj ruam no, lub xov tooj ntawm tes thiab cov nucleus tau tawg qhib kom tso DNA sab hauv thiab muaj ob txoj hauv kev los ua qhov no.
Yog li ntawd, dab tsi yog 4 kauj ruam ntawm DNA rho tawm?
Plaub kauj ruam yog siv los tshem tawm thiab ntxuav cov DNA ntawm tus so ntawm tes
- Lysis.
- Dej nag.
- Ntxuav.
- Kev rov kho dua.
Tom qab, lo lus nug yog, DNA rho tawm li cas? DNA rho tawm yog ib txoj kev niaj hnub siv los cais DNA los ntawm cov nucleus ntawm cov cell. Thaum ib qho dej khov-txias cawv ntxiv rau kev daws ntawm DNA , cov DNA precipitates tawm ntawm kev daws. Yog muaj txaus DNA nyob rau hauv cov tshuaj, koj yuav pom ib tug stringy dawb pawg.
Cov lus nug tseem yog, koj yuav rho tawm DNA los ntawm dos li cas?
Qhov kev sim
- Chop lub dos.
- Ncuav cov nqaij tenderizer tov (100 ml) rau hauv 250 mL beaker thiab kub rau 60ºC hauv dej da dej.
- Ntxiv 50 g ntawm tws dos rau nqaij tenderizer tov.
- Tshem lub beaker los ntawm da dej thiab tam sim ntawd muab tso rau hauv dej khov rau tsib feeb.
- Ncuav qhov sib tov rau hauv ib lub blender.
Dab tsi tshuaj siv rau hauv DNA rho tawm?
SDS, CTAB, phenol, chloroform, isoamyl cawv, Triton X100, guanidium thiocyanate, Tris thiab EDTA yog ntau yam. tshuaj siv hauv kev daws teeb meem raws li DNA rho tawm txoj kev.
Pom zoo:
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