Vim li cas h2so4 nres cov tshuaj tiv thaiv enzymatic?
Vim li cas h2so4 nres cov tshuaj tiv thaiv enzymatic?

Video: Vim li cas h2so4 nres cov tshuaj tiv thaiv enzymatic?

Video: Vim li cas h2so4 nres cov tshuaj tiv thaiv enzymatic?
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Cov sulfuric acid txo qis pH theem ntawm cov tshuaj, uas ua rau catalase rau denature los ntawm tau txais hydrogen ions thiab nws nres tus tshuaj tiv thaiv tam sim ntawd. 5. twv seb cov nyhuv txo qhov kub thiab txias yuav muaj nyob rau ntawm tus nqi ntawm enzyme kev ua si. Txo qhov kub thiab txias yuav ua rau tshuaj tiv thaiv kom qeeb.

Tsis tas li ntawd, sulfuric acid ua dab tsi rau catalase?

Sulfuric acid muaj inhibiting nyhuv catalase ua haujlwm vim nws ua rau pH qib hauv cov tshuaj txo qis qis. Acid Cov kev daws teeb meem ua rau cov qauv protein ntawm cov enzyme kom nce H + ions ua rau nws mus rau denature.

Ib tug kuj yuav nug, dab tsi yog kev daws teeb meem? STOP Solution yog ib tug tswv daws siv los txiav tawm cov tshuaj tiv thaiv peroxidase / TMB rau ELISA daim ntawv thov. Lub TMB substrate reacts nrog immobilized horseradish peroxidase (HRP) conjugated thib ob cov tshuaj tiv thaiv los tsim cov xiav daws . Xim siv yog qhov qhia txog qib ntsuas.

Kuj paub, thaum twg yuav tsum tau tso tseg Elisa cov tshuaj tiv thaiv?

Nres tshuaj: Nres Kev daws yog siv los txiav cov enzyme substrate tshuaj tiv thaiv rau ELISA daim ntawv thov tom qab tau txais qhov xav tau xim siv uas yog qhov qhia txog qib ntsuas. Rau eg. Lub TMB substrate reacts nrog immobilized horseradish peroxidase (HRP) conjugated antibodies los tsim cov tshuaj xiav.

Vim li cas peb thiaj li tso tseg cov tshuaj tiv thaiv colorimetric nyob rau theem kawg ntawm Elisa?

Lub ntsiab yog vim li cas yog uas nyiam tshaj plaws colorimetric assays, thaum kawg stong signals yuav tshaj qhov linear ntau ntawm amplification. Thaum qhov no tshwm sim, qhov dej tsis tuaj yeem muab piv rau qhov tseeb. Cov nres daws yog mus quench tshuaj tiv thaiv nyob rau hauv nruab nrab ntawm no linear theem ntawm amplification thiaj li tshuaj tiv thaiv nres.

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