Video: Vim li cas h2so4 nres cov tshuaj tiv thaiv enzymatic?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov sulfuric acid txo qis pH theem ntawm cov tshuaj, uas ua rau catalase rau denature los ntawm tau txais hydrogen ions thiab nws nres tus tshuaj tiv thaiv tam sim ntawd. 5. twv seb cov nyhuv txo qhov kub thiab txias yuav muaj nyob rau ntawm tus nqi ntawm enzyme kev ua si. Txo qhov kub thiab txias yuav ua rau tshuaj tiv thaiv kom qeeb.
Tsis tas li ntawd, sulfuric acid ua dab tsi rau catalase?
Sulfuric acid muaj inhibiting nyhuv catalase ua haujlwm vim nws ua rau pH qib hauv cov tshuaj txo qis qis. Acid Cov kev daws teeb meem ua rau cov qauv protein ntawm cov enzyme kom nce H + ions ua rau nws mus rau denature.
Ib tug kuj yuav nug, dab tsi yog kev daws teeb meem? STOP Solution yog ib tug tswv daws siv los txiav tawm cov tshuaj tiv thaiv peroxidase / TMB rau ELISA daim ntawv thov. Lub TMB substrate reacts nrog immobilized horseradish peroxidase (HRP) conjugated thib ob cov tshuaj tiv thaiv los tsim cov xiav daws . Xim siv yog qhov qhia txog qib ntsuas.
Kuj paub, thaum twg yuav tsum tau tso tseg Elisa cov tshuaj tiv thaiv?
Nres tshuaj: Nres Kev daws yog siv los txiav cov enzyme substrate tshuaj tiv thaiv rau ELISA daim ntawv thov tom qab tau txais qhov xav tau xim siv uas yog qhov qhia txog qib ntsuas. Rau eg. Lub TMB substrate reacts nrog immobilized horseradish peroxidase (HRP) conjugated antibodies los tsim cov tshuaj xiav.
Vim li cas peb thiaj li tso tseg cov tshuaj tiv thaiv colorimetric nyob rau theem kawg ntawm Elisa?
Lub ntsiab yog vim li cas yog uas nyiam tshaj plaws colorimetric assays, thaum kawg stong signals yuav tshaj qhov linear ntau ntawm amplification. Thaum qhov no tshwm sim, qhov dej tsis tuaj yeem muab piv rau qhov tseeb. Cov nres daws yog mus quench tshuaj tiv thaiv nyob rau hauv nruab nrab ntawm no linear theem ntawm amplification thiaj li tshuaj tiv thaiv nres.
Pom zoo:
Vim li cas cov tshuaj tiv thaiv kev zom zaub mov hu ua hydrolysis cov tshuaj tiv thaiv?
Thaum lub sij hawm zom mov, piv txwv li, decompositionreactions zom cov zaub mov loj rau hauv cov me me los ntawm kev ntxiv cov dej molecules. Hom tshuaj no hu ua hydrolysis. Raws li dej absorbs heatenergy, qee lub zog yog siv los rhuav tshem hydrogenbonds
Cov tshuaj twg yog ib txwm tsim los ntawm cov tshuaj tiv thaiv neutralization?
Cov tshuaj tiv thaiv acid-base neutralization ib txwm ua rau ntsev. Qee zaum dej tsuas yog tsim cov tshuaj tiv thaiv uas muaj cov ntses bass thaj tsam muaj zog. Yog li cov lus teb yog ntsev
Cov tshuaj tiv thaiv zoo li cas thaum cov tshuaj nkag mus rau hauv cov hlab ntsha?
Cov tshuaj tiv thaiv 'systemic' tshwm sim thaum cov tshuaj nkag mus rau hauv cov hlab ntsha los ntawm daim tawv nqaij, qhov muag, qhov ncauj, lossis lub ntsws
Cov tshuaj tiv thaiv dab tsi yog qhov tshuaj tiv thaiv neutralization?
Neutralization yog ib hom tshuaj tiv thaiv nyob rau hauv uas muaj zog acid thiab muaj zog puag react nrog ib leeg los tsim dej thiab ntsev
Dab tsi yog tshuaj lom neeg cov tshuaj tiv thaiv thiab cov tshuaj tiv thaiv lub cev?
Qhov sib txawv ntawm cov tshuaj tiv thaiv lub cev thiab cov tshuaj tiv thaiv tshuaj yog muaj pes tsawg leeg. Hauv cov tshuaj tiv thaiv tshuaj, muaj kev hloov pauv ntawm cov tshuaj hauv nqe lus nug; nyob rau hauv ib qho kev hloov ntawm lub cev muaj qhov sib txawv ntawm qhov tsos, tsis hnov tsw, los yog cov duab yooj yim ntawm cov qauv ntawm cov teeb meem yam tsis muaj kev hloov pauv hauv cov ntsiab lus