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Video: Yuav ua li cas koj pom wavelength los ntawm absorbance?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Muab l los ntawm c thiab muab faib A los ntawm cov khoom los daws cov molar absorptivity. Piv txwv li: Siv lub cuvette nrog qhov ntev ntawm 1 cm, koj ntsuas qhov absorbance Kev daws teeb meem nrog qhov concentration ntawm 0.05 mol / L. Cov absorbance ntawm a wavelength 280nm yog 1.5.
Kuj paub yog, koj ua li cas xam wavelength los ntawm absorbance?
x l x c, qhov twg A yog tus nqi ntawm lub teeb absorbed los ntawm tus qauv rau ib tug muab wavelength ,? yog cov molar absorptivity, l yog qhov kev ncua deb uas lub teeb mus los ntawm kev daws, thiab c yog qhov concentration ntawm absorbing hom ib chav tsev ntim.
Ib yam li ntawd, lub wavelength ntawm qhov siab tshaj plaws absorbance yog dab tsi? 560nm ua
Kuj nug, ua li cas koj pom lub wavelength ntawm lub spectrophotometer?
1 Teb
- Kev ua haujlwm wavelength yog xaiv los ntawm kev txheeb xyuas cov spectrogram A(λ).
- Qhov twg ν yog qhov zaus ntawm EM yoj, c qhov ceev ntawm lub teeb thiab h lub Plank tas li.
- Lub spectrophotometer ntsuas qhov transmittance T (qhov sib piv ntawm kis ϕ thiab xwm txheej flux ϕ0 qhia raws li lub hwj chim) ntawm lub teeb beam los ntawm cov qauv ntawm tes.
Dab tsi yog chav tsev ntawm absorbance?
AU
Pom zoo:
Yuav ua li cas koj pom joules los ntawm wavelength?
Qhov sib npaug rau kev txiav txim siab lub zog ntawm photon ofelectromagnetic hluav taws xob yog E = h ν qhov twg E yog lub zog hauv Joules, h yog Planck qhov tas li, 6.626 × 10 − 34J ν (pronounced 'noo') yog qhov zaus. Koj tau txais lub wavelength λ (hais lambda) hauv nanometers, tab sis tsis yog qhov zaus
Yuav ua li cas koj pom molarity los ntawm absorbance?
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