Dab tsi koj yuav tsum paub txog Crispr?

2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36

CRISPR yog ib lub ntsiab lus rau "Clustered Regularly Interspaced Short Palindromic Repeats." CRISPR genome engineering thev naus laus zis ua rau cov kws tshawb fawb tau yooj yim thiab meej kho cov DNA ntawm txhua lub genome. Nyob rau hauv qhov, lub CRISPR palindromic repeats ua lub luag haujlwm tseem ceeb hauv kev tiv thaiv kab mob microbial.

Ib yam li ib tug yuav nug, dab tsi yog qhov xav tau rau Crispr?

Engineered CRISPR systems muaj ob yam: ib qho kev qhia RNA (gRNA lossis sgRNA) thiab a CRISPR -associated endonuclease (Cas protein). Lub gRNA yog ib qho luv luv hluavtaws RNA tsim los ntawm kev sib tw scaffold tsim nyog rau Cas-binding thiab tus neeg siv-txhais ∼20 nucleotide spacer uas txhais lub hom phiaj genomic los hloov.

Kuj Paub, Crispr yog tam sim no siv rau dab tsi? CRISPR tsuas yog ib qho cuab yeej tshiab rau kev ua cov txheej txheem me ntsis-tsawg-tshiab. Hauv University of Pennsylvania sim, nws hais tias, CRISPR yog ua siv rau ua kom cov kab mob tiv thaiv kab mob hu ua CAR-T hlwb uas xav tias yuav zoo dua ntawm kev tawm tsam cov qog uas tsis tu ncua cov hlwb.

Kuj Paub, Crispr yog dab tsi thiab vim li cas nws tseem ceeb?

CRISPR thev naus laus zis yog qhov cuab yeej yooj yim tab sis muaj zog rau kev kho genomes. Nws tso cai rau cov kws tshawb fawb tau yooj yim hloov DNA sib lawv liag thiab hloov cov noob muaj nuj nqi. Nws muaj ntau daim ntawv thov muaj xws li kho cov kab mob caj ces, kho thiab tiv thaiv kev kis kab mob thiab txhim kho cov qoob loo.

Crispr ua haujlwm ib kauj ruam li cas?

Kauj ruam 1) Kev hloov kho - DNA los ntawm tus kab mob nkag mus rau hauv cov ntu luv uas tau muab tso rau hauv CRISPR raws li cov tshiab spacers. Kauj ruam 2) Production ntawm CRISPR RNA – CRISPR rov ua dua thiab spacers hauv cov kab mob DNA tau txais kev hloov pauv, cov txheej txheem ntawm kev luam DNA rau hauv RNA (ribonucleic acid).