Video: Puas yog gal4 protein nyob rau hauv cov poov xab ua tau zoo lossis tsis zoo ntawm GAL noob?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov Gal4 transcription factor yog ib zoo regulator ntawm noob kev qhia ntawm galactose -induced noob . Qhov no protein ntau sawv cev rau ib tsev neeg fungal loj ntawm kev hloov pauv, Gal4 tsev neeg, uas suav nrog ntau dua 50 tus tswv cuab hauv lub poov xab Saccharomyces cerevisiae e.g. Oaf1, Pip2, Pdr1, Pdr3, Leu3.
Khaws qhov no hauv kev xav, dab tsi yog gal4 gene?
Gal4 yog transcriptional activator uas khi rau UAS enhancer sequences pom nyob rau hauv DNA. Nws ces recruits transcription machinery mus rau qhov chaw kom induce noob kev qhia. Yog li, noob and siRNA encoded downstream of UAS sequence is only expressed when Gal4 yog qhia.
gal80 yog dab tsi? GAL80 / YML051W Txheej txheem cej luam GAL80 encodes ib tug transcriptional repressor koom nyob rau hauv kev cai transcriptional teb rau galactose (2). Nyob rau hauv lub xub ntiag ntawm galactose, Gal3p cuam tshuam nrog Gal80p, uas relieves inhibition ntawm Gal4p thiab ua rau GAL noob qhia (6).
Tom qab ntawd, ib tus tuaj yeem nug, gal7 thiab gal10 tswj hwm li cas?
Tag nrho cov galactose structural noob (GAL1, GAL10 , GAL7 , GAL2) yog kev sib koom tes tswj nyob rau theem ntawm kev hloov pauv hauv cov lus teb rau galactose los ntawm Gal4p, Gal80p, thiab Gal3p (4, 6, thiab tshuaj xyuas hauv 7).
Puas yog gal4 yog tus txhawb nqa?
GAL4 /UAS is a binary system with two main components: The poov xab transcription factor GAL4 (tshwj xeeb hauv cov ntaub so ntswg thiab / lossis lub sijhawm tshwj xeeb qauv) ua kom cov transgenes nyob rau hauv kev tswj ntawm UAS (sab sauv activating sequence) txhawb nqa , yog li ua rau spatiotemporal tshwj xeeb transgene qhia.
Pom zoo:
Dab tsi yog txhais los ntawm cov noob tseem ceeb thiab cov noob caj noob ces?
(Nyob rau hauv cov ntsiab lus ntawm caj ces, ib qho tseem ceeb tshaj plaws yog ib qho uas yog phenotypically qhia hauv heterozygotes). Ib qho tseem ceeb tshaj plaws yog tawm tsam qhov tsis zoo uas tshwm sim tsuas yog thaum muaj ob daim ntawv theej ntawm cov noob. (Nyob rau hauv cov ntsiab lus genetic, ib tug recessive zoo yog ib tug uas yog phenotypically qhia tsuas yog nyob rau hauv homozygotes)
Candida puas yog cov poov xab?
Cov poov xab. Cov poov xab yog cov fungi uas loj hlob raws li ib leeg hlwb, tsim cov menyuam ntxhais los ntawm budding (cov budding poov xab) los yog los ntawm binary fission (cov fission poov xab). Dimorphic fungus Candida albicans uas tuaj yeem ua tus kab mob tseem ceeb ntawm tib neeg. ib co ntawm cov nplooj saum npoo poov xab
Yog vim li cas sodium poov tshuaj twj tso kua mis suav hais tias yog ib qho kev thauj mus los uas cov kev taw qhia yog cov sodium thiab poov tshuaj tau pumped?
Sodium-Potassium Pump. Kev thauj mus los yog lub zog xav tau cov txheej txheem ntawm kev siv cov molecules thiab ions hla daim nyias nyias 'nce toj' - tiv thaiv kev sib tw gradient. Txhawm rau txav cov molecules tawm tsam lawv cov concentration gradient, yuav tsum muaj cov khoom siv protein
Puas yog cov ntsiab lus uas muaj cov tshuaj lom neeg zoo sib xws yuav pom nyob rau tib lub sijhawm lossis hauv tib pab pawg piav qhia koj cov lus teb?
Qhov no yog vim hais tias cov tshuaj muaj zog nyob ntawm tsis muaj valence electrons. Raws li nyob rau hauv ib pawg tag nrho cov ntsiab lus muaj tib yam tsis muaj valence electron uas yog vim li cas lawv muaj cov tshuaj zoo sib xws tab sis nyob rau hauv ib lub sij hawm tsis muaj valence electron sib txawv uas yog vim li cas lawv txawv nyob rau hauv chemical zog
Ntau npaum li cas ntawm cov pej xeem hauv ntiaj teb nyob ntawm cov kab ke roob rau tag nrho lossis qee qhov ntawm lawv cov dej?
Roob yog lub ntiaj teb "dej yees," muab 60-80% ntawm tag nrho cov dej tshiab rau peb ntiaj chaw. Tsawg kawg ib nrab ntawm lub ntiaj teb cov pej xeem nyob ntawm roob ecosystem kev pabcuam kom muaj sia nyob - tsis yog dej xwb, tab sis kuj muaj zaub mov thiab lub zog huv