Puas yog gal4 protein nyob rau hauv cov poov xab ua tau zoo lossis tsis zoo ntawm GAL noob?
Puas yog gal4 protein nyob rau hauv cov poov xab ua tau zoo lossis tsis zoo ntawm GAL noob?

Video: Puas yog gal4 protein nyob rau hauv cov poov xab ua tau zoo lossis tsis zoo ntawm GAL noob?

Video: Puas yog gal4 protein nyob rau hauv cov poov xab ua tau zoo lossis tsis zoo ntawm GAL noob?
Video: Playful Kiss - Playful Kiss: Full Episode 1 (Official & HD with subtitles) 2024, Kaum ib hlis
Anonim

Cov Gal4 transcription factor yog ib zoo regulator ntawm noob kev qhia ntawm galactose -induced noob . Qhov no protein ntau sawv cev rau ib tsev neeg fungal loj ntawm kev hloov pauv, Gal4 tsev neeg, uas suav nrog ntau dua 50 tus tswv cuab hauv lub poov xab Saccharomyces cerevisiae e.g. Oaf1, Pip2, Pdr1, Pdr3, Leu3.

Khaws qhov no hauv kev xav, dab tsi yog gal4 gene?

Gal4 yog transcriptional activator uas khi rau UAS enhancer sequences pom nyob rau hauv DNA. Nws ces recruits transcription machinery mus rau qhov chaw kom induce noob kev qhia. Yog li, noob and siRNA encoded downstream of UAS sequence is only expressed when Gal4 yog qhia.

gal80 yog dab tsi? GAL80 / YML051W Txheej txheem cej luam GAL80 encodes ib tug transcriptional repressor koom nyob rau hauv kev cai transcriptional teb rau galactose (2). Nyob rau hauv lub xub ntiag ntawm galactose, Gal3p cuam tshuam nrog Gal80p, uas relieves inhibition ntawm Gal4p thiab ua rau GAL noob qhia (6).

Tom qab ntawd, ib tus tuaj yeem nug, gal7 thiab gal10 tswj hwm li cas?

Tag nrho cov galactose structural noob (GAL1, GAL10 , GAL7 , GAL2) yog kev sib koom tes tswj nyob rau theem ntawm kev hloov pauv hauv cov lus teb rau galactose los ntawm Gal4p, Gal80p, thiab Gal3p (4, 6, thiab tshuaj xyuas hauv 7).

Puas yog gal4 yog tus txhawb nqa?

GAL4 /UAS is a binary system with two main components: The poov xab transcription factor GAL4 (tshwj xeeb hauv cov ntaub so ntswg thiab / lossis lub sijhawm tshwj xeeb qauv) ua kom cov transgenes nyob rau hauv kev tswj ntawm UAS (sab sauv activating sequence) txhawb nqa , yog li ua rau spatiotemporal tshwj xeeb transgene qhia.

Pom zoo: