Video: Lub hom phiaj ntawm lysis yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
A lis buffer yog ib qho kev daws teeb meem tsis siv rau cov lub hom phiaj ntawm kev rhuav tshem cov hlwb siv rau hauv kev sim tshuaj molecular biology uas txheeb xyuas cov labile macromolecules ntawm cov hlwb (xws li western blot rau cov protein, lossis rau DNA rho tawm). Lysis buffers tuaj yeem siv rau ntawm ob qho tib si tsiaj thiab cov ntaub so ntswg.
Hauv qhov no, lub hom phiaj ntawm cell lysis yog dab tsi?
Lysis hais txog kev rhuav tshem ntawm cell , feem ntau los ntawm kab mob, enzymic, lossis osmotic mechanisms uas cuam tshuam nws txoj kev ncaj ncees. Cov kua dej uas muaj cov ntsiab lus ntawm lysed hlwb hu ua " lysate ". Cell lysis yog siv los rhuav qhib hlwb kom tsis txhob shear rog uas yuav denature lossis degrade rhiab proteins thiab DNA.
Ib yam li ntawd, 2 lub ntsiab lus ntawm cov tshuaj lysis yog dab tsi? Cov formulation muaj xws li ob ionic detergents thiab ib tug nonionic detergent nyob rau hauv Tris tsis: 25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP40, 1% sodium deoxycholate thiab 0.1% sodium dodecyl sulfate (SDS).
Yog li ntawd, lub hom phiaj ntawm EDTA yog dab tsi hauv cov tshuaj nuclei lysis?
EDTA yog ib qho tshuaj ntxiv uas muaj ntau yam haujlwm xws li protease inhibition thiab tiv thaiv oxidative puas tsuaj. Tris yog lwm yam khoom siv ntxiv rau tsis pub pH thiab tiv thaiv cov protein denaturation.
Lub luag haujlwm ntawm lysis tsis nyob hauv DNA extraction yog dab tsi?
Qhov tseem ceeb ntawm lysis buffer rau DNA rho tawm : Nws lyses lub nuclear membrane raws li ib tug cell membrane. Nws tswj cov pH thaum lub sij hawm DNA rho tawm . Lysis buffer tswj kev ncaj ncees ntawm cov DNA (tiv thaiv DNA los ntawm lis ) Nws sib cais DNA los ntawm lwm cov khib nyiab ntawm tes.
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