Cov txheej txheem:

PCR voj voog yog dab tsi?
PCR voj voog yog dab tsi?

Video: PCR voj voog yog dab tsi?

Video: PCR voj voog yog dab tsi?
Video: Leng Yang - Yog Vim Dab Tsi (Official Full Song | Nkauj Tawm Tshiab) 2020/05/24 2024, Kaum ib hlis
Anonim

Polymerase chain reaction , los yog PCR , yog ib qho txheej txheem los ua ntau daim ntawv luam ntawm ib cheeb tsam DNA tshwj xeeb hauv vitro (hauv lub raj kuaj tsis yog kab mob). Hauv PCR , cov tshuaj tiv thaiv yog rov ua dua los ntawm kev hloov pauv ntawm qhov kub thiab txias, uas tso cai rau ntau cov ntawv luam ntawm lub hom phiaj ntawm cheeb tsam yuav raug tsim.

Ib yam li ntawd, koj tuaj yeem nug, dab tsi yog txhais los ntawm PCR voj voog?

Polymerase chain reaction , los yog PCR , yog cov txheej txheem kuaj siv los ua ntau daim ntawv luam ntawm ib ntu ntawm DNA. Tom qab synthesis thiab thaum kawg ntawm thawj lub voj voog , txhua ob-stranded DNA molecule muaj ib tug tshiab thiab ib tug qub DNA strand.

Kuj Paub, 3 kauj ruam ntawm PCR yog dab tsi? PCR yog ua raws li peb kauj ruam yooj yim uas yuav tsum tau ua rau txhua qhov DNA synthesis cov tshuaj tiv thaiv: (1) denaturation ntawm tus qauv rau hauv ib qho strands; (2) annealing ntawm primers rau txhua tus thawj strand rau tshiab strand synthesis; thiab (3) txuas ntxiv ntawm DNA tshiab strands los ntawm primers.

4 kauj ruam ntawm PCR yog dab tsi?

Cov kauj ruam koom nrog hauv Polymerase Chain Reaction hauv DNA Sequence

  • Kauj Ruam 1: Denaturation los ntawm Thaum tshav kub kub: Thaum tshav kub kub yog feem ntau ntau tshaj 90 degrees Celsius ntawm cais ob-stranded DNA rau hauv ob qho tib si strands.
  • Kauj Ruam 2: Annealing Primer rau Target Sequence:
  • Kauj Ruam 3: Extension:
  • Kauj Ruam 4: Xaus ntawm Thawj PGR voj voog:

PCR yog dab tsi thiab nws ua haujlwm li cas?

Polymerase chain reaction ( PCR ) yog ib txoj kev siv dav hauv molecular biology kom sai sai ua ntau lab mus rau billions ntawm cov ntawv luam ntawm cov qauv DNA tshwj xeeb tso cai rau cov kws tshawb fawb coj tus qauv DNA me me thiab nthuav dav rau ntau qhov txaus los kawm kom ntxaws.

Pom zoo: