Video: H&E siv rau dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Hematoxylin thiab eosin stain los yog hematoxylin thiab eosin stain (feem ntau abbreviated li: H&E stain los yog HE stain ) yog ib qho ntawm cov ntaub so ntswg loj stains siv nyob rau hauv histology. Cov stain qhia txog cov qauv dav dav thiab kev faib tawm ntawm cov hlwb thiab muab cov ntsiab lus dav dav ntawm cov qauv ntawm cov ntaub so ntswg.
Cov lus nug tseem yog, H&E siv rau dab tsi?
Feem ntau siv staining system hu ua H&E (Haemotoxylin thiab Eosin). H&E muaj ob dyes haemotoxylin thiab eosin. Eosin yog ib qho acidic dye: nws raug nqi tsis zoo (cov qauv dav dav rau acidic dyes yog: Na+xim-). Nws stains yooj yim (los yog acidophilic) qauv liab lossis liab.
Qhov thib ob, hematoxylin thiab eosin ua haujlwm li cas? Haematoxylin nyob rau hauv complex nrog txhuas ntsev yog cationic thiab ua raws li ib tug yooj yim dye. Nws raug nqi zoo thiab tuaj yeem hnov mob tsis zoo, basophilic cell Cheebtsam, xws li nucleic acids hauv lub nucleus. Cov stain xiav raws li qhov tshwm sim. Eosin yog anionic thiab ua raws li acidic dye.
Tsis tas li ntawd, lub hauv paus ntsiab lus ntawm H&E staining yog dab tsi?
Txoj Cai Lub acidic tivthaiv ntawm cov hlwb muaj affinity rau cov xim yooj yim thiab cov ntsiab lus tseem ceeb ntawm cov hlwb muaj affinity rau acidic dye. Hauv hematoxylin thiab eosin stain , hematoxylin stains acidic ib feem ntawm lub cell, i.e. Nucleus. Yog li hematoxylin hu ua nuclear stain.
H thiab E cwj mem yog dab tsi?
Hav! H & E txawv xaum , qhov twg H sawv cev rau Hematoxylin thiab E sawv cev rau Eosin. Ob qho no txawv xaum muab cov xim sib txawv, H imparts/stains blusish xim rau lub nuclei thiab E muab pinkish stain so. Eosin xaum.
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