Video: Kuv yuav tshem tawm H&E stains li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Yaug nrog 0.25% hydrochloric acid (HCl) rau 2-5 vib nas this lossis 1% acid cawv (1ml Conc HCl hauv 100ml ethanol) rau tshem tawm tshaj stain los ntawm cov swb, Tom qab ntawd khaws cov slides hauv dej ntws rau 3 feeb rau blueing.
Ib yam li ntawd, koj tuaj yeem nug, koj yuav ua li cas tshem tawm eosin stains?
Anhydrous isopropyl cawv muaj qhov tsis zoo solubility rau eosin ua , thiab yog li ntawd tsis yaug tawm qhov dhau eosin ua . Diluted isopropyl cawv (70%, 95%) tuaj yeem tshem tawm tshaj stain.
Ib tug kuj yuav nug, dab tsi eosin stain? Eosin yog feem ntau dye rau stain cytoplasm hauv histology. Nws yog ib qho acidic dye uas khi rau cov khoom tseem ceeb ntawm cov cell, feem ntau cov proteins nyob hauv cytoplasm. Nws muab cov xim liab liab uas sib txawv ntawm qhov tsaus ntuj xiav nuclear hematoxylin staining (Fig. 1.3B).
Kuj Paub, H&E staining ua li cas?
H&E muaj ob dyes haemotoxylin thiab eosin. Eosin yog ib qho acidic dye: nws raug nqi tsis zoo (cov qauv dav dav rau acidic dyes yog: Na+xim-). Nws stains yooj yim (los yog acidophilic) qauv liab lossis liab. Yog li lub nucleus yog ua qias neeg ntshav hauv daim duab hauv qab no, los ntawm H&E staining.
Vim li cas eosin stain cytoplasm?
Eosin yog anionic thiab ua raws li acidic dye. Nws yog qhov tsis zoo thiab tuaj yeem cuam tshuam nrog cov txiaj ntsig zoo, acidophilic Cheebtsam hauv cov ntaub so ntswg, xws li cov amino pawg hauv cov proteins hauv lub cev. cytoplasm . Cov no stain liab raws li qhov tshwm sim.
Pom zoo:
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