Dab tsi yog qhov absorbance ntawm spectrophotometer?
Dab tsi yog qhov absorbance ntawm spectrophotometer?

Video: Dab tsi yog qhov absorbance ntawm spectrophotometer?

Video: Dab tsi yog qhov absorbance ntawm spectrophotometer?
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Absorbance yog ib qho kev ntsuas ntawm qhov ntau ntawm lub teeb absorbed los ntawm ib tug qauv. Nws tseem hu ua optical density, extinction, los yog decadic nqus tau . Yog hais tias tag nrho lub teeb dhau los ntawm ib tug qauv, tsis muaj ib tug absorbed, yog li cov nqus tau yuav yog xoom thiab kis tau tus mob yuav 100%.

Kuj tseem paub yog, dab tsi yog chav tsev rau absorbance hauv spectrophotometer?

Qhov tseeb chav tsev kev ntsuas ntawm nqus tau tau tshaj tawm raws li absorbance units , sau AU. Absorbance yog ntsuas siv a spectrophotometer , uas yog ib lub cuab tam uas ci dawb lub teeb los ntawm ib yam khoom uas yaj nyob rau hauv ib tug hnyav thiab ntsuas tus npaum li cas ntawm lub teeb uas cov khoom absorbs nyob rau hauv lub wavelength uas teev.

Ib tug kuj yuav nug, ua li cas lub spectrometer ntsuas absorbance? A spectrophotometer ntsuas lub zog I ntawm lub nqaj hla dhau los ntawm tus qauv thiab muab piv rau qhov nyiaj no lossis qhov siv zog ntawm lub zog rau qhov siv Io lossis qhov tshwm sim lub zog ntawm lub nqaj. Qhov tshwm sim hu ua transmittance T.

Kuj tseem paub, dab tsi yog absorbance hauv colorimeter?

A colorimeter yog lub teeb-sensitive ntaus ntawv siv rau kev ntsuas lub transmittance thiab nqus tau ntawm lub teeb dhau los ntawm cov qauv ua kua. Cov cuab yeej ntsuas qhov kev siv zog lossis qhov siab ntawm cov xim uas tsim los ntawm kev qhia ib qho reagent rau hauv cov tshuaj.

Dab tsi yog units ntawm absorbance?

Absorbance yog ntsuas hauv absorbance units (Au), uas cuam tshuam rau kev xa tawm raws li pom hauv daim duab 1. Piv txwv li, ~ 1.0Au yog sib npaug rau 10% transmittance, ~ 2.0Au yog sib npaug rau 1% transmittance, thiab lwm yam nyob rau hauv ib tug logarithmic sib.

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