Video: Puas muaj lwm txoj hauv kev los ntxuav cov khoom PCR?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Rau cov ntawv thov uas xav tau PCR ntxuav los yog validation ntawm PCR tshwm sim, nyob ntawd yog ob txoj kev feem ntau ua raws li: PCR khoom Kev rho tawm siv ib sab, thiab gel ua kom huv los ntawm agarose gel.
Ntawm no, hom chromatography yog siv los ntxuav PCR cov khoom?
Txhawm rau ua kom huv ntawm PCR cov khoom, qhov loj me tsis suav nrog chromatography yog siv. Qhov no ntxiab me me molecules xws li cov protein, primers , thiab nucleotides thaum loj molecules zoo li PCR cov khoom loj dhau los nkag rau hauv cov hlaws dai thiab hla hauv kem mus rau hauv cov hlab ntim.
Tsis tas li, koj ua li cas ntxuav DNA? Yeej, koj ua tau ua kom huv koj DNA kuaj los ntawm lysating koj lub xov tooj ntawm tes thiab / lossis cov qauv ntaub so ntswg siv cov txheej txheem tsim nyog tshaj plaws (kev cuam tshuam kev siv tshuab, kev kho tshuaj lom neeg lossis kev zom zaub mov enzymatic), cais cov nucleic acids los ntawm nws cov kab mob thiab precipitating nws nyob rau hauv ib tug haum tsis haum tshuaj.
Tsis tas li ntawd, vim li cas peb thiaj li ntxuav cov khoom PCR?
Kev huv huv DNA los ntawm a PCR Cov tshuaj tiv thaiv feem ntau tsim nyog rau kev siv qis, thiab pab tshem tawm cov enzymes, nucleotides, primers thiab tsis khoom. Feem ntau siv txoj hauv kev siv cov kab sib tw uas muaj cov silica matrix uas DNA tuaj yeem xaiv tau khi rau hauv lub xub ntiag ntawm chaotropic ntsev.
Vim li cas thiaj yuav tsum tau ntxuav DNA?
Peb huv si nce DNA los ntawm cov tshuaj aqueous kom tshem tawm cov ntsev tsis muaj ntsev, enzymes lossis lwm yam khoom uas tuaj yeem cuam tshuam rau cov ntawv thov hauv qab. Piv txwv xws li cov tshuaj tiv thaiv PCR huv si nce, huv si tom qab kev txwv digests thiab huv si nce ntawm genomic los yog plasmid DNA kis nrog cellular proteins / khib nyiab.
Pom zoo:
Puas yog nws muaj tseeb tias nyob rau hauv kev thauj mus los ntawm kev txav ntawm cov khoom hla ib daim nyias nyias yuav tsum muaj zog?
Hauv kev thauj mus los passive, kev txav ntawm cov khoom hla ib daim nyias nyias yuav tsum muaj zog. _ Muaj tseeb_ 5. Endocytosis yog ib txoj kev uas ib lub cell membrane nyob ib puag ncig thiab siv cov khoom los ntawm ib puag ncig. Ib daim nyias nyias uas tso cai rau qee cov ntaub ntawv dhau los qhia txog kev xaiv permeability
Txoj kev faib cov ntaub ntawv twg muab cov ntaub ntawv sib npaug ntawm cov ntaub ntawv lossis cov kev tshuaj xyuas hauv txhua chav kawm cov ntaub ntawv?
Quantile. txhua chav kawm muaj cov yam ntxwv sib npaug. Ib qho kev faib tawm ntau yog zoo haum rau cov ntaub ntawv faib tawm. Quantile muab tib tus lej ntawm cov ntaub ntawv muaj nuj nqis rau txhua chav kawm
Puas yog qhov ceev yog ib txoj kev txhim khu kev qha ntawm kev txheeb xyuas tag nrho cov khoom tsis paub?
Koj tuaj yeem txheeb xyuas cov khoom tsis paub los ntawm kev ntsuas nws qhov ntom thiab sib piv koj cov txiaj ntsig rau cov npe paub qhov ntom ntom. Qhov ntom = loj / ntim. Xav tias koj yuav tsum txheeb xyuas cov hlau tsis paub. Koj tuaj yeem txiav txim siab qhov loj ntawm cov hlau ntawm qhov ntsuas
Yuav ua li cas cov khoom siv caj ces hauv txhua lub xov tooj tshiab tsim los ntawm kev faib tawm ntawm tes sib piv nrog cov khoom siv caj ces hauv lub xov tooj qub?
Mitosis tshwm sim nyob rau hauv ob lub nuclei uas zoo tib yam rau thawj nucleus. Yog li, ob lub hlwb tshiab tsim tom qab kev faib tawm ntawm tes muaj tib yam khoom siv caj ces. Thaum lub sij hawm mitosis, chromosomes condense los ntawm chromatin. Thaum saib nrog lub microscope, chromosomes pom nyob rau hauv lub nucleus
Puas muaj qhov chaw nruab nrab ntawm cov khoom hauv cov khoom?
Feem ntau, cov khib nyiab yog denser dua cov kua, uas yog denser dua gases. Cov khoom hauv cov kua feem ntau tseem kov tab sis muaj qee qhov chaw nruab nrab ntawm lawv. Cov pa roj muaj qhov sib txawv loj ntawm lawv