Dab tsi yog tus qauv rau transformation efficiency?
Dab tsi yog tus qauv rau transformation efficiency?

Video: Dab tsi yog tus qauv rau transformation efficiency?

Video: Dab tsi yog tus qauv rau transformation efficiency?
Video: Tsis Tau Txaus Koj | Kong Chue (Official Music Video) 2024, Kaum ib hlis
Anonim

Nws tuaj yeem suav tau los ntawm kev faib cov naj npawb ntawm cov hloov pauv tau zoo los ntawm tus nqi ntawm DNA siv thaum lub sijhawm a kev hloov pauv txheej txheem.

Yog li ntawd, dab tsi yog qhov kev hloov pauv ib txwm muaj?

Qhov no yog muab faib los ntawm tus nqi ntawm DNA siv nyob rau hauv lub kev hloov pauv thiab nthuav tawm raws li kev hloov pauv ib microgram ntawm DNA. Kev hloov pauv tau zoo nruab nrab ntawm 10^6 thiab 10^9 sawv cev rau li qub rau cov E. coli hlwb muaj peev xwm.

Tom qab ntawd, lo lus nug yog, qhov kev hloov pauv ntawm E coli yog dab tsi? Transformation efficiency yog txhais raws li tus naj npawb ntawm colony forming units (cfu) uas yuav tsim los ntawm hloov pauv 1 µg ntawm plasmid rau hauv ib lub ntim ntawm cov hlwb muaj peev xwm. Lub sij hawm yog me ntsis yuam kev nyob rau hauv uas 1 µg ntawm plasmid yog tsis tshua muaj hloov.

Ib yam li ntawd, dab tsi cuam tshuam rau kev hloov pauv kev ua tau zoo?

Cov yam uas cuam tshuam rau transformation efficiency yog cov mob ntawm kab mob , cov kab mob colony theem ntawm kev loj hlob, lub muaj pes tsawg leeg ntawm transformation mix, thiab cov qhov loj thiab lub xeev ntawm txawv teb chaws DNA.

Puas yog cov kab mob hloov pauv tau zoo?

Tej yam cuam tshuam Transformation Efficiency Cov kev hloov pauv tshuaj tiv thaiv yog muaj txiaj ntsig thaum <10ng ntawm DNA siv. pUC19 DNA (0.1ng) yog tsim los tswj. Supercoiled DNA yog feem ntau muaj txiaj ntsig rau kev hloov pauv piv rau linear lossis ssDNA uas muaj transformation efficiency ntawm <1%.

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