Yuav ua li cas khaws agarose gel?
Yuav ua li cas khaws agarose gel?

Video: Yuav ua li cas khaws agarose gel?

Video: Yuav ua li cas khaws agarose gel?
Video: Yuav tawm ntawm txoj kev txom nyem tau li cas? 2024, Kaum ib hlis
Anonim

9. Yog tias koj tsis muaj sijhawm txaus los mus txuas ntxiv mus Agarose gel electrophoresis , khw tus gel nyob rau hauv lub thawv, npog nrog 25 ml ntawm 1 x TAE tsis nyob rau hauv ib lub hnab yas kaw nyob rau hauv chav tsev kub rau 1 hnub, los yog nyob rau hauv lub tub yees (4 ° C) mus txog rau 1 lub lis piam ua ntej siv lawv. Nco ntsoov sau koj lub hnab yas.

Ntawm no, yuav ua li cas khaws agarose gel?

Agarose gel muaj ib khaws cia Lub neej ntawm kwv yees li 3 - 4 lub lis piam yog tias nws tov nrog cov ntsiab lus ntawm cov tshuaj tsis haum thiab nws yuav tsum muab cia rau hauv qhov tsaus ntuj ntawm qhov kub ntawm ib ncig 4. 0C. Nws yog lub teeb heev rhiab heev thiab yuav tsum tsis txhob cia nyob rau hauv lub teeb rau ntau tshaj 3 teev.

Ib sab saum toj no, nws siv sijhawm ntev npaum li cas rau agarose gel kom khov? Txhua npuas tuaj yeem raug thawb tawm ntawm lub qhov dej los yog mus rau sab / ntug ntawm lub gel nrog ib tug pipette taub. Qhov chaw tshiab nchuav gel ntawm 4 ° C rau 10-15 mins LOSSIS cia zaum ntawm chav tsev kub rau 20-30 mins, kom txog thaum nws tag nrho. khov kho.

Kuj paub, koj puas tuaj yeem tso ib gel rau hauv tsis tau thaum hmo ntuj?

Gel Extract DNA los ntawm agarose gel tau yuav muab tso tawm mus tsis kawg. Sim khaws cia gel hlais hauv lub tub yees hmo ntuj , los yog txawm melting daim nyob rau hauv tsis pub thiab khov nws ntawm -20 ° C lossis -80 ° C.

Koj puas tuaj yeem ua agarose gel nrog dej?

Siv dej es tsis txhob buffer rau lub gel los yog khiav tsis Cov gels yog cam khwb cia thiab khiav siv TAE lossis TBE tsis. Yog koj ua siv dej ,koj gel yuav yaj sai sai tom qab siv voltage rau lub electrophoresis chav tsev. TAB, TBE, and dej yog txhua yam kev daws teeb meem; Yog li ntawd, xyuas daim ntawv lo ntawm lub thawv thaum teeb tsa.

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