Video: Dab tsi cuam tshuam rau RF qhov tseem ceeb hauv daim ntawv chromatography?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
yam uas cuam tshuam rau tus nqi Rf yog:-• Cov kuab tshuaj system thiab nws muaj pes tsawg leeg. Kub. Qhov zoo ntawm daim ntawv. Nyob deb ntawm qhov uas kuab tshuaj khiav.
Ib yam li ntawd, tib neeg nug, dab tsi cuam tshuam rau daim ntawv chromatography?
Khaws cia yam muaj nuj nqis nyob rau hauv nyias txheej chromatography cuam tshuam los ntawm absorbent, hnyav, lub chromatography phaj nws tus kheej, daim ntawv thov txheej txheem thiab qhov kub thiab txias ntawm cov kuab tshuaj thiab phaj.
Cov kuab tshuaj sib txawv puas cuam tshuam rau RF qhov tseem ceeb? Lub zog eluting ntawm cov kuab tshuaj nce nrog polarity. Non-polar compounds txav mus rau lub phaj sai tshaj plaws (siab dua Rf nqi ), whereas polar substances mus txog TLC phaj maj mam los yog tsis tag (qis Rf nqi ).
Ua li no, dab tsi yog RF yam hauv daim ntawv chromatography?
Cov Rf Tus nqi yog txhais raws li qhov piv ntawm qhov kev ncua deb txav los ntawm cov kuab tshuaj (piv txwv li cov dye lossis pigment nyob rau hauv kev sim) thiab qhov kev ncua deb txav los ntawm cov kuab tshuaj (lub npe hu ua Solvent pem hauv ntej) raws tus ntawv , qhov twg ob qhov kev ncua deb yog ntsuas los ntawm cov hauv paus chiv keeb los yog Daim Ntawv Thov Baseline, uas yog lub ntsiab lus uas tus qauv yog
Tus nqi RF puas nyob ntawm hom chromatography?
Nws tag nrho nyob ntawm ntawm hom chromatography . Yog tias koj tab tom ua ntawv chromatography , TLC lossis HPTLC, feem ntau Retention factor ( Rf nqi ) yog siv raws li nws yog qhov piv ntawm qhov kev ncua deb mus los ntawm cov kuab tshuaj thiab cov kuab tshuaj pem hauv ntej. Rf tus nqi tau yuav pom pom qhov zoo li no los yog los ntawm derivatisation (yog tias tsim nyog).
Pom zoo:
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Thaum ib qho kev quab yuam sab nraud yog exerted rau ntawm cov kua uas muaj, qhov tshwm sim siab yog kis sib npaug thoob plaws hauv cov kua. Yog li txhawm rau kom dej ntws, dej xav tau qhov sib txawv siab. Cov kav dej kuj tuaj yeem cuam tshuam los ntawm cov kua dej, cov yeeb nkab loj, qhov kub thiab txias (cov kav dej khov), cov kua dej ntom ntom