Video: Koj puas tuaj yeem autoclave phenol liab?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Phenol liab thaum ntxiv ua ib feem rau cov ntaub so ntswg kab lis kev cai media, ua tau ua autoclaved . Ib qho kev daws teeb meem ua tau yog tsim los ntawm dissolving 0.1 g ntawm phenol liab Hauv 14.20 ml ntawm 0.02 N NaOH thiab diluted rau 250 ml nrog dej deionized.
Hauv qhov no, cov xim ntawm phenol liab nyob rau hauv ib qho kev daws teeb meem yog dab tsi?
Kev daws ntawm phenol liab yog siv los ua pH qhia, feem ntau hauv kab lis kev cai ntawm tes. Nws cov xim nthuav tawm ib qho kev hloov maj mam los ntawm daj (λmax = 443 nm) mus red (λmax = 570 nm) tshaj pH ntau 6.8 txog 8.2. Tshaj pH 8.2, phenol liab hloov a ci liab ( fuchsia ) xim. thiab yog txiv kab ntxwv-liab.
Tom qab, lo lus nug yog, lub hom phiaj ntawm phenol liab yog dab tsi? Phenol Liab Broth yog general- lub hom phiaj Qhov nruab nrab ntsuas qhov sib txawv feem ntau yog siv los sib txawv gram negative enteric kab mob. Nws muaj peptone, phenol liab (ib pH qhia), ib lub raj Durham, thiab ib qho carbohydrate. Phenol liab yog lub ntsuas pH uas hloov daj hauv qab pH ntawm 6.8 thiab fuchsia saum pH ntawm 7.4.
Kuj nug, nws txhais li cas thaum phenol liab tig daj?
Phenol liab yog pH qhov taw qhia uas yog daj ntawm pH hauv qab 6.8 thiab liab ntawm pH saum toj no 7.4 nrog sib txawv ntxoov los ntawm daj rau liab nruab nrab ntawm cov qib pH. Yog tias qhov ntsuas tau tig daj hauv lub raj mis no txhais tau tias Nws tau dhau los ua paug nrog qee yam uas ua rau pH ntau acidic thiab coj pH qis dua 6.8.
Dab tsi ua rau xim hloov hauv phenol liab?
Cov phenol liab hloov xim thaum koj tshuab rau nws, vim koj tab tom qhia cov pa roj carbon dioxide rau kev sib tov. Phenol liab hloov kom daj nyob rau hauv pH qis dua 7, yog li qhov kev daws teeb meem tig daj yog qhov qhia txog acidic (qis dua 7 pH).
Pom zoo:
Vim li cas cov phenol liab tig liab?
Tshaj pH 8.2, phenol liab hloov xim liab (fuchsia) xim. thiab yog txiv kab ntxwv-liab. Yog tias pH nce ntxiv (pKa = 1.2), cov proton los ntawm pawg ketone ploj, ua rau daj, tsis zoo them ion denoted li HPS& rho tawm;
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