Collagen stain xim dab tsi?
Collagen stain xim dab tsi?

Video: Collagen stain xim dab tsi?

Video: Collagen stain xim dab tsi?
Video: How to stain collagen - The Van Gieson staining protocol 2024, Kaum ib hlis
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Collagen fibers stain ntsuab los yog xiav nrog Masson's trichrome stain. Cov leeg thiab keratin yuav yog liab.

Kuj paub yog, dab tsi yog trichrome stain rau?

Trichrome staining yog siv los pom cov ntaub so ntswg sib txuas, tshwj xeeb tshaj yog collagen, hauv cov ntaub so ntswg. Nyob rau hauv ib tug standard Masson's Trichrome Cov txheej txheem, collagen yog stained xiav, nuclei yog stained tsaus xim av, nqaij nqaij yog stained liab, thiab cytoplasm yog stained liab.

Ib tug kuj yuav nug, eosin stain collagen? Eosin stains lub cytoplasm thiab lwm yam qauv xws li extracellular matrix xws li collagen nyob rau hauv mus txog tsib ntxoov liab. Cov eosinophilic (cov khoom uas yog ua qias neeg los ntawm eosin ua ) cov qauv feem ntau yog tsim los ntawm intracellular lossis extracellular proteins.

Tsis tas li ntawd, PAS puas stain collagen?

Nws yog lub hauv paus ntawm lub PAS stain . PAS stains carbohydrates thiab carbohydrate nplua nuj macromolecules xim liab (magenta). Mucus nyob rau hauv cov hlwb thiab cov ntaub so ntswg, hauv qab daim nyias nyias, thiab txhuam ciam teb ntawm lub raum tubules thiab cov hnyuv me thiab loj Reticular fibers (piv txwv li. collagen ) nyob rau hauv cov ntaub so ntswg connective thiab pob txha mos.

Dab tsi xim ua cov leeg tau txais hauv Masson trichrome staining?

Masson Trichrome Staining Weigert's hlau hematoxylin stains lub nuclei dub, Biebrich scarlet-acid fuchsin stains cytoplasm thiab cov leeg fibers hauv liab thiab tom qab kho nrog phosphotungstic thiab phosphomolybdic acid, collagen yog ua qias neeg hauv xiav nrog aniline xiav. Kev pleev xim txheej txheem: 1.

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